Journal:

Article Title: Kr?ppel-Like Factor 5 Protects Against Dextran Sulfate Sodium-Induced Colonic Injury by Promoting Epithelial Repair in Mice

doi: 10.1053/j.gastro.2010.10.061

Figure Lengend Snippet: Induction of KLF5 with DSS treatment is dependent on ERK1/2 signaling. (A) Detection of ERK1/2 and NF-κB activation by Western blotting, using pooled lysates from WT mice treated with 3.5% DSS over a 5 day time course (n=3). (B) Caco-2 cells were treated with 3% DSS for up to 8 hrs, and lysates were examined by Western blotting. (C) Caco-2 cells were pretreated with inhibitors of MEK/ERK (U0126) or NF-κB (Bay 11-7082), and treated for 5 hrs with 3% DSS. Lysates were examined by Western blotting.

Article Snippet: For inhibitor studies, Caco-2 cells were pretreated for 10 min prior to addition of DSS with U0126 at 50 μM (Cell Signaling Technology; Beverly, MA) or Bay 11-7082 at 20μM (Calbiochem; San Diego, CA).

Techniques: Activation Assay, Western Blot